Western blot analysis and immunoprecipitation Total cells lysates for immunoblotting or immunoprecipitation were lysed with buffer (0.1%Triton-100, 50 mM Tris-Cl, pH 7.5, 0.1% NP-40, 150 mM NaCl, 0.1 M EDTA) supplemented with a cocktail of phosphate and proteinase inhibitors for 30 min at 4 C
Significantly enhances Phase II detox in the liver, aiding in the safe elimination of heavy metals, environmental toxins, and hormonal byproducts
Considering the typical sample size of 812 participants in each group for most studies with a similar experimental design, in the present study our sample size of 25 in each group can be considered a strength, rather than a limitation
10.1016/j.cmet.2016.11.005 146 LiY.SetoE
This is because the glutathione molecule is delicate and easily destroyed during digestion